Electrical and Chemical Control of Surfaces for DNA Immobilization and Hybridization

摘要

We present the design of two biointerfaces on a SiO_2 substrate for single stranded DNA (ssDNA) immobilization using either covalent grafting or electrostatic adsorption. The influence of the type of biointerface on the rate of diffusion-limited hybridization reaction with complementary ssDNA from a solution is studied. Patterning of the biointerface functionalization layers and the scaling down of the reaction volumes to μL range is demonstrated. The use of externally applied electric field pulses is shown to accelerate the hybridization reaction kinetics to the sub-ms time scale.