Variations of Hsp60 and HSF-1 in Primary Myocardial Cells of Rats Under Various Durations of in vitro Heat Stress

摘要

The relationship between the heat stress induced variations of protective Hsp60 and its regulating factor HSF-1 expression in the heat-stressed primary myocardial cells of neonatal rats in in vitro was studied by using enzyme detections.immunoblotting and qPCR.The enzymes elevations displayed injuries of myocardial cells after heat exposure at 42℃.The enzymes elevated at 42℃ heat exposure and displayed injuries of myocardial cells.Hsp60 expression level was fluctuated during heat stress and decreased significantly after 20 min,then elevated at 120 min and reduced at 360 min of heat stress.The damages of myocardial cells characterized by enzyme elevations and over consumption of Hsp60 concerned to the functional disorder of myocardial cells at early stage of heat stress.However the significant induction results of hsp60 mRNA levels (P＜0.01 )from the beginning up t0 240 min of heat stress were not consistent with the classic regulatory mechanisms.hsf-1 mRNA level was significantly increased from 10 min of heat stress,but HSF-1 protein was not simultaneously increased,indicating that HSF-1 is not the sole regulator of Hsp60 expression.