DNA Strand Breaks and DNA-protein Crosslinks of Mast Cell Induced by Butyl Benzyl Phthalate

摘要

This experiment was about the effect of Butyl benzyl phthalate on DNA strand breaks and the DNA-protein crosslinks in cells. Mouse mast cells cultured in vitro were exposed to different concentrations of BBP (0、4、20、100μmol·L-1) for 1h. Comet assay and KCl-SDS assay were used to measure the DNA strand breaks and DNA-protein crosslinks. Results showed that, all of the BBP treatment groups (4、20、100μmol·L-1) couldThis experiment was about the effect of Butyl benzyl phthalate on DNA strand breaks and the DNA-protein crosslinks in cells. Mouse mast cells cultured in vitro were exposed to different concentrations of BBP (0、4、20、100μmol·L-1) for 1h. Comet assay and KCl-SDS assay were used to measure the DNA strand breaks and DNA-protein crosslinks. Results showed that, all of the BBP treatment groups (4、20、100μmol·L-1) could cause DNA damage, compared with the control group. But, BBP could induce DNA strand breaks at the lower dose (4μmol·L-1), and could primarily induce DPC at the middle dose (20μmol·L-1) and the higher dose (100μmol·L-1). Cause DNA damage, compared with the control group. But, BBP could induce DNA strand breaks at the lower dose (4μmol·L-1), and could primarily induce DPC at the middle dose (20μmol·L-1) and the higher dose (100μmol·L-1).