Groundwater constitutes the largest terrestrial freshwater ecosystem,but still belongs to the least explored one on earth.The capability of groundwater ecosystems to recover from pollutions(such as fuel spills)is a vital concern for environmental scientists.The growing use of ethanol as transportation biofuel is increasing the likelihood of encountering ethanol in current and future fuel releases.However,no studies have comprehensively investigated the temporal variability in microbial community structure and the long-term evidence for contaminant elimination and groundwater geochemistry evolution throughout the whole life cycle of a biofuel release,from initial plume expansion through its stabilization and eventual disappearance.
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