摘要：
目的 研究芍药总苷对6MV-X射线放射性食管炎模型大鼠的影响.方法 取健康雄性Wistar大鼠,用6MV-X射线单次照射建立放射性食管炎模型,空白组大鼠17只不接受射线照射.按照体重将造模成功大鼠随机分为4组,每组17只:模型组、对照组和低、高2个剂量实验组.模型组和空白组给予0.9％ NaCl 2 mL,tid;对照组给予西药合剂(0.9％ NaCl 250 mL+利多卡因3.2mL+地塞米松1.6 mg+硫酸庆大霉素5.12万单位)2 mL tid;低、高2个剂量实验组分别给予2,6 mg·mL-芍药总苷溶液2 mL,tid,均连续给药8d.用光学显微镜观察食管组织病理变化并评分,用酶联免疫吸附法测定血清Ⅲ型前胶原(PC Ⅲ)、转化生长因子β1(TGF-β1)水平,用蛋白质免疫印迹法检测食管组织神经生长因子(NGF)、酪氨酸蛋白激酶A(TrkA)蛋白、P75蛋白表达水平.结果 给药后,空白组、模型组、对照组和低、高2个剂量实验组食管组织病理变化程度评分分别为0,(4.24±0.58),(2.15±0.26),(1.08±0.15),(0.53±0.07)分,这5组的血清PC Ⅲ水平分别为(40.73±5.24),(90.48±10.38),(71.23±8.72),(58.46±7.66),(49.36±6.12) μg·L-1,这5组的食管组织NGF蛋白表达水平分别为0.54±0.07,1.32±0.18,1.13±0.12,0.84 ±0.11,0.69±0.08,组间比较差异均有统计学意义(均P＜0.05).结论 芍药总苷能够减轻6MV-X射线放射性食管炎模型大鼠的病理损伤程度,降低血清PCⅢ、TGF-β1水平,防治食管纤维化,其作用可能与降低NGF及其受体TrkA蛋白和P75蛋白的表达水平有关.%Objective To analyze the effect of total glucosides of peony (TGP) on nerve growth factor(NGF) and its receptor in 6MV-X ray radiation induced esophagitis model rats.Methods The male Wistar rats were selected,and radiation esophagitis rat model were established by 6MV-X ray single irradiation,the model rats were randomly divided into model group,control group,experimental-L group,experimental-H group,each with 17 rats;another 17 rats in blank group did not receive radiation.The model group and blank group were treated with sodium chloride injection 2 mL,tid;the control group was treated with western medicine mixture (sodium chloride injection 250 mL + lidocaine 3.2 mL + dexamethasone 1.6 mg + gentamicin sulphate 51.2 × 104 u) 2 mL,tid;the experimental-L group and experimental-H group were treated with 2,6 mg · mL-1 TGP solution 2 mL,tid.All rats were given the drug continuously for 8 days.The score of pathological changes of esophagus were observed by optical microscope.The serum levels of type Ⅲ procollagen (PC Ⅲ) and transforming growth factor β1 (TGF-β1) were detected by enzyme-linked immunosorbent assay.The expression levels of nerve growth factor(NGF),tyrosine protein kinase A (TrkA)protein,P75 protein were detected by Western blotting.Results After treatment,the score of pathological changes of esophagus in blank group,model group,corntrol group,experimental-L group,experimental-H group were respectively 0,(4.24 ± 0.58),(2.15 ± 0.26),(1.08 ± 0.15) and (0.53 ± 0.07) point,serum PC Ⅲ levels in the 5 groups were respectively (40.73 ± 5.24),(90.48 ± 10.38),(71.23 ± 8.72),(58.46 ± 7.66) and (49.36-± 6.12) μg · L-1,the expression levels of NGF in esophageal tissue in the 5 groups were respectively 0.54 ± 0.07,1.32 ± 0.18,1.13 ± 0.12,0.84 ± 0.11 and 0.69 ± 0.08,there were significant differences between groups (all P ＜ 0.05).Conclusion TGP can reduce the pathological damage degree of 6MV-X ray radiation induced esophagitis model rats,reduce serum PC Ⅲ and TGF-β1 levels,prevent and cure esophageal fibrosis,which may be related to decrease the expression levels of NGF and its receptor TrkA protein and P75 protein.
