DOUBLE-ENDED LIBRARY LABEL COMPOSITION AND APPLICATION THEREOF IN MGI SEQUENCING PLATFORM

摘要

The invention provides a double-end library tags composition and application thereof in MGI sequencing platform. The double-end library tags composition includes a plurality of 5'-end library tags and a plurality of 3'-end library tags, the lengths of the plurality of 5'-end library tags are all the same, the lengths of the plurality of 3'-end library tags are all the same,and in the double-end library tags composition, the occurrences of each base at the same position are also all the same. The use of optimized double-end library tags for sequencing data splitting can solve the crosstalk problems caused during synthesis, experimental process and machine sequencing. And by controlling the lengths of the 5' end library tags are all the same, the lengths of the 3' end library tags are all the same, and occurrences of each base at the same position are all the same, we can obtain multiple libraries with good base-balanced double-end tags, and when the multiple libraries are pooled for sequencing, the double-end tags are read more accurately, improving library sequencing splitting rate effectively.