Substrate Sequences for the RNAi-Mediated Regulation of Genomic and Sub-genomic Viral RNAs

摘要

Provided is a method for identifying an RNAi sequence, comprising the steps of screening for one or more 7-mers from a gRNA; selecting a first number of the one or more 7-mers; characterizing one or more hit sites for each of the one or more 7-mers; calculating a hit per genomic region for each of the 7-mers; selecting a second number of the 7-mers; creating a frequency matrix for one or more 15-mers associated to each of the 7-mers; generating one or more generated 15-mers for each of the 7-mers; characterizing hits in the gRNA for each of the one or more generated 15-mers; calculating a summary index based on a cumulative hit frequency and a length; and selecting the RNAi sequences based on one or more generated features and one or more structural features.