Detection of structural remodeling in cell, extracellular matrix and tissue microenvironments

摘要

The method of the invention predicts the physiological state of the subject. The composition comprising the activity sensor is introduced into the body of the subject. The activity sensor contains multiple reporters that are sensitive to cleavage when processed by the body during the reconstruction of the extracellular matrix. Cleavage may indicate enzymatic activity in the extracellular matrix. The signal detected by the activity sensor can predict the physiological state. The sample is taken from the subject and the released reporter is detected in the sample. The onset of a subject's physiological state, which may be a disease characterized by inflammation or atrophy, can be diagnosed on the basis of detected released reporters.