CRISPR SYSTEM AND USE THEREOF IN PREPARATION OF SEVERE-IMMUNODEFICIENCY CLONED PIG NUCLEAR DONOR CELLS WITH MULTIPLE GENES JOINTLY KNOCKED OUT

摘要

Provided are a CRISPR system and the use thereof in the preparation of severe-immunodeficiency cloned pig nuclear donor cells with multiple genes jointly knocked out. Provided are a method for preparing severe-immunodeficiency pig-derived recombinant cells with four genes, i.e. the ADA gene, the DQA gene, the DRA gene and the IL2RG gene, jointly knocked out; a CRISPR system; a combination of sgRNAs; a combination of plasmids; and a kit, wherein the combination of sgRNAs is composed of sgRNAADA-g7 as shown in SEQ ID NO: 11, sgRNADQA-gn2 as shown in SEQ ID NO: 21, sgRNADRA-g1 as shown in SEQ ID NO: 28 and sgRNAIL2RG-g7 as shown in SEQ ID NO: 40, and can be used for preparing recombinant cells, immunodeficiency animal models and immunodeficiency animal cell models.