METHOD FOR ANALYSING A BIOLOGICAL SAMPLE COMPRISING DETERMINATION OF THE SPATIAL DISTRIBUTION OF BIOMASS ALONG THE OPTICAL AXIS

摘要

A method of analyzing a biological sample comprising biological agents and disposed in an analysis receptacle in a field of view of a holographic imaging system defining an acquisition focal plane, comprising, for each of a plurality of measurement times: acquiring (S02a) a plurality of holographic images of the biological sample at different respective positions of the acquisition focal plane, and, from each acquired holographic image, determining (S02b) a value of a biomass parameter representative of the quantity of biological agents at the position of the acquisition focal plane, the method comprising constructing (S03) a distribution indicator from values of the biomass parameter at the same measurement time for a plurality of positions of the acquisition focal plane, and providing, among the analysis results (S05), a representation of the distribution of the biomass of biological agents, derived from at least one distribution indicator at a measurement time.