Cellular cancer strains and animal models for developing primary liver cancer using them

摘要

PROBLEM TO BE SOLVED: To provide a hepatic cell carcinoma cell line or a cell cancer cell line which is a cholangiocellular carcinoma cell line that can increase the malignancy in the living body of an experimental animal, such as an immunodeficient mouse, and can engraft to an animal such as a wild type mouse, a method for establishing same, and an animal model of onset of primary liver cancer which is a hepatic cell carcinoma or cholangiocellular carcinoma.SOLUTION: The establishment of a cell carcinoma as a hepatocellular carcinoma cell line or a cholangiocellular carcinoma cell line a comprises: a step of making an animal, to which hepatitis C has been genetically introduced, ingest arteriosclerotic high cholesterol food and high neutral fat food; a step of generating hepatic carcinoma in the liver of the animal; a step of collecting liver cancer cells from the generated liver cancer tissue; and a step of culturing the collected liver cancer cells. The animal model of onset of primary liver cancer that is hepatocellular carcinoma or cholangiocellular carcinoma is obtained by forming a tumor by establishing the cell cancer cell line and thereafter, engrafting the cell cancer cell line to the liver of an animal to which the cell cancer is to be engrafted with the same cell line, a different cell line or a different type.SELECTED DRAWING: None