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SCALABLE METHODS FOR OBTAINING RECOMBINANT VECTOR BASED ON ADENO-ASSOCIATED VIRUS (AAV) IN SYSTEM OF SERUM-FREE SUSPENSION CELL CULTURE, SUITABLE FOR CLINICAL USE
SCALABLE METHODS FOR OBTAINING RECOMBINANT VECTOR BASED ON ADENO-ASSOCIATED VIRUS (AAV) IN SYSTEM OF SERUM-FREE SUSPENSION CELL CULTURE, SUITABLE FOR CLINICAL USE
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机译:在无血清悬浮细胞培养系统中获得基于腺相关病毒(AAV)的重组载体的可扩展方法,适合临床使用
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FIELD: biotechnology.;SUBSTANCE: methods and compositions for cell transfection using plasmids are described. In certain implementations, methods and compositions are described, in which the transfection efficiency is significantly increased by bringing transduced cells into contact with polyethyleneimine (PEI) that does not contain nucleic acid during transfection. Therapeutically applicable vectors based on adeno-associated viruses are also described, obtained using described methods and compositions.;EFFECT: invention expands the arsenal of therapeutic means.;58 cl, 6 dwg, 2 tbl, 5 ex
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机译:领域:生物技术。;物质:描述了使用质粒进行细胞转染的方法和组合物。在某些实施方案中,描述了方法和组合物,其中通过使转导细胞在转染期间与不含核酸的聚乙烯亚胺(PEI)接触,显著提高了转染效率。还描述了使用所述方法和组合物获得的基于腺相关病毒的治疗用载体。;效果:发明扩大了治疗手段的范围。;58 cl,6图纸,2 tbl,5 ex
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