A scalable method for producing recombinant adeno-associated virus (AAV) vectors in a serum-free suspension cell culture system suitable for clinical use

摘要

Methods and compositions for transfecting cells with plasmids are disclosed. In certain embodiments, disclosed are methods and compositions that significantly increase transfection efficiency by contacting transducing cells with polyethylenimine (PEI) without nucleic acid during the transfection step. ing. Also disclosed are therapeutically useful adeno-associated viral vectors made according to the disclosed methods and compositions. [Selection] Figure 3A