METHOD FOR DETERMINING MEDICINAL SENSITIVITY OF NONSMALL CELL LUNG CANCER IN VITRO

摘要

FIELD: medicine.;SUBSTANCE: invention relates to medicine and relates to a method for determining the drug sensitivity of non-small cell lung cancer in vitro by culturing cancer cells in the presence of a cytostatic, where sections of tumor tissue with a thickness of 300-500 mcm obtained from the primary tumor are cultured, while the sections are placed in a 24-well plate, according to one section in each well with a nutrient medium and a cytostatic, some of the wells are left as control, where the wells with control contain a nutrient medium without a cytostatic, cultured in a CO2 incubator with constant stirring, at the end of cultivation, the sections are fixed and stored until cDNA is isolated and until determined in their expression of RNA markers, with an increase in the expression of RNA markers in comparison with the control sample, drug sensitivity to the investigated cytostatic is determined.;EFFECT: invention provides an individual selection of cytostatic drug therapy using sections of tumor tissue, the correct and timely implementation of cytostatic drug therapy.;1 cl, 1 ex