Establishment of an 'In Vitro Cell-Based System' to Assay Radiation Sensitivity in Breast Cancer; Annual rept. 1 May 2007-30 Apr 2008

摘要

The purpose of this proposal is to develop an 'in vitro cell-based system' to study radioresistance in sporadic breast cancer. By performing colony formation assays in response to X-ray treatment we have shown that there is a wide range of sensitivities to this form of treatment. Interestingly, we have also observed that p53 status does not accurately predict response to radiation treatment. Using correlation studies, we have identified novel predictors of IR resistance/sensitivity. We have sensitized two breast cancer cell lines to X-ray treatment by using RNAi-based technology to inhibit Artemis function. In the future, these observations may have a significant clinical impact on the way radiation oncologists treat breast cancer patients.