METHOD FOR REPROGRAMMING UMBILICAL CORD MESENCHYMAL STEM CELLS INTO LIVER CELLS AND LIVER ORGANOID PREPARED THEREFROM

摘要

Provided is a method for reprogramming umbilical cord mesenchymal stem cells into liver cells. The method comprises obtaining umbilical cord mesenchymal stem cells; pretreating the umbilical cord mesenchymal stem cells for 2 days; using a hepatic differentiation-inducing composition to conduct a hepatic differentiation of the pretreated umbilical cord mesenchymal stem cells for 7 days; after the hepatic differentiation induction treatment, using a first maturation inducing composition to conduct a first maturation induction of umbilical cord mesenchymal stem cells for 7 days to obtain liver cells; and using a second maturation inducing composition to conduct a second maturation induction of the obtained liver cells for 12-20 days to obtain functional liver cells. A type of liver organoids: acellular matrix hydrogel, stem cells and liver cells. The stem cells and liver cells are distributed in the acellular matrix hydrogel. Cultivation time is prolonged, and the induction efficiency is significantly increased by adding the additional step of second maturation induction.