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METHODS FOR GENERATING, EVALUATING, GENE EDITING AND CLONING PLURIPOTENT STEM CELLS COMPRISING A LETHAL HAPLOTYPE
METHODS FOR GENERATING, EVALUATING, GENE EDITING AND CLONING PLURIPOTENT STEM CELLS COMPRISING A LETHAL HAPLOTYPE
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机译:产生,评价,基因编辑和克隆多能干细胞的方法,所述多能干细胞包含致死的单倍型
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摘要
The invention includes a method of evaluating a bovine embryo by fertilizing an egg obtained from a first bovine heterozygote of a recessive lethal haplotype with sperm cells obtained from a second bovine heterozygote of the recessive lethal haplotype; producing the embryo from the fertilized egg, wherein the embryo is homozygous for the lethal haplotype; establishing a cell culture from the embryo; collecting a plurality of cultured cells; and obtaining omics data, comprising one or more features, from the plurality of cultured cells.
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