首页> 外国专利> Method for Producing y-aminobutyric Acid (GABA) by Fermentation of Native Strain from Raw Material of Sayram Ketteki

Method for Producing y-aminobutyric Acid (GABA) by Fermentation of Native Strain from Raw Material of Sayram Ketteki

机译:通过从Sayram Ketteki的原料发酵生物菌株生产y-氨基丁酸(GABA)的方法

摘要

The present invention discloses a method for producing γ-aminobutyric acid (GABA) by fermentation of native strain from raw material of Sayram Ketteki, comprising the following steps: dissolving the cow's milk in the normal saline for dilution to obtain diluents with different gradients first, coating the diluents in solid media for culture respectively, isolating single colonies and storing them in a glycerin cryopreservation tube for cryopreservation respectively; next, inoculating them in liquid medium for culture respectively, and inoculating them in fermentation medium for culture for culture after activation. The content of GABA product obtained by the present invention can reach 450 μg/mL.
机译:本发明公开了一种通过Sayram Ketteki的原料发酵产生γ-氨基丁酸(GABA)的方法,包括以下步骤:将牛奶溶解在正常盐水中以稀释,首先用不同梯度获得稀释剂, 分别在固体培养基中涂覆稀释剂,分别分离单片菌落并将它们储存在甘油冷冻保存管中分别用于冷冻保存; 接下来,将它们分别在液体培养基中分别用于培养物中,并在激活后将它们接种在发酵培养基中用于培养培养物。 通过本发明获得的GABA产品的含量可达到450μg/ mL。

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