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PERIPHERAL BLOOD TCR MARKER FOR CERVICAL CANCER, AND TEST KIT AND APPLICATION THEREOF

机译:宫颈癌外周血TCR标志物,以及其试剂盒及其应用

摘要

Disclosed in the present invention are a peripheral blood TCR marker for a cervical cancer, and a test kit and an application thereof. The marker comprises at least one of proteins shown in sequences SEQ ID NO.1-100. In the present invention, on the basis of a high-throughput sequencing method, only a small amount of peripheral blood needs to be taken to extract an RNA; an immune map library is established by means of processing of a sample; and then, a characteristic TCR sequence in peripheral blood of the cervical cancer is first determined by means of high-throughput sequencing and TCR data analysis, and a test result of a sample to be tested is then compared with the characteristic TCR sequence, thereby determining whether there is a cervical cancer. According to the present invention, a large amount of cervical cancer-specific TCR sequences can be simultaneously compared, and compared with individually testing one or several markers, the present invention has higher specificity and accuracy, thereby improving the diagnosis efficiency.
机译:本发明公开的是用于宫颈癌的外周血TCR标记,以及测试试剂盒及其应用。标记物包含序列ID NO.1-00中所示的蛋白质中的至少一种。在本发明中,在高通量测序方法的基础上,仅需要少量的外周血来提取RNA;通过处理样品建立免疫图库;然后,首先通过高通量测序和TCR数据分析确定宫颈癌外周血的特征TCR序列,然后将要测试的样品的测试结果与特征TCR序列进行比较,从而确定是否存在宫颈癌。根据本发明,可以同时比较大量的宫颈癌特异性TCR序列,并与单独测试一个或多个标记进行比较,本发明具有更高的特异性和精度,从而提高了诊断效率。

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