IMPROVED PROCESS FOR DNA INTEGRATION USING RNA-GUIDED ENDONUCLEASES

摘要

There is disclosed an improved, safer and commercially efficient process for developing genetically engineered cells. More specifically, there is disclosed a process comprises introducing a donor DNA construct, a guide RNA, and an RNA-guided nuclease with the host cells to be transfected; and introducing the three components into the host cell. There is further disclosed donor DNA constructs designed for inserting genetic constructs into a defined genomic site of a host cell, such as a target site of the TRAC gene. Further, the present disclosure provides host cells transfected with genetic constructs that lacks viral vectors that can present a safety concern. The disclosure provides for more efficient and more cost-effective process for engineering T ceils to express CAR or DAR constructs.