The present invention relates to a bio-ink composition comprising gelatin, hydroxyapatite and glycerol, and a method for manufacturing a three-dimensional structure for bone regeneration using the same, and the problem of clogging a printer nozzle is solved It is possible to produce a continuous and stable three-dimensional structure, and it maintains excellent mechanical properties even after glycerol is removed after production of the structure, and at the same time has the effect of exhibiting excellent cell proliferation, growth and differentiation ability. It is expected to be useful.
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