METHOD FOR DETECTING SARS-CoV-2 VIRUS RNA AT ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD

摘要

FIELD: genetic engineering ;SUBSTANCE: group of inventions relates to the field of genetic engineering and biotechnology. A method for detecting SARS-CoV-2 virus RNA, a specific oligonucleotide and a set for use in a method for detecting SARS-CoV-2 virus RNA are proposed. The method for detecting SARS-CoV-2 virus RNA uses guide RNAs represented by SEQ ID NO: 1-4 and specific oligonucleotides for preliminary amplification represented by SEQ ID NO: 5-8. ;EFFECT: inventions make it possible to efficiently detect the RNA of the SARS-CoV-2 virus after carrying out specific amplification of fragments of the genome of this virus and ensures the detection of single copies of the RNA of the SARS-CoV-2 virus.;5 cl, 16 dwg, 5 tbl, 5 ex