Manufacturing method of acellular dermal matrix derived from human

摘要

The present invention relates to a method for preparing a human-derived acellular dermal matrix capable of simultaneously performing de-epidermal, de-fat and de-cellularization, Specifically (i) preparing a human-derived skin tissue that has not undergone a separate de-epidermal and de-fat process; (ii) treating the skin tissue with a hypotonic solution containing a surfactant; and (iii) washing the skin tissue obtained in step (ii) with an isotonic solution; It is characterized in that it includes. The process according to the present invention shortens the time of the cell-free dermal processing process by performing one-step de-epidermal, de-fat and de-celling when a hypotonic solution containing an ionic surfactant is treated in human-derived skin tissue. and the convenience of the process can be greatly increased. In addition, the process according to the present invention minimizes tissue damage, reduces the residual amount of detergent in the tissue, and has excellent safety against cytotoxicity.