METHOD OF ENCAPSULATION OF AN ACTIVE PROTEIN USING ELECTRODEPOSITION TECHNIQUES, AN IMMUNOMODULATING COMPOSITION CONTAINING THE ACTIVE PROTEIN AND A POLYMER, AND ITS USE FOR THE PRODUCTION OF A PHARMACEUTICAL COMPOSITION FOR THE TREATMENT OF ATOPIC DERMATITIS IN HUMANS

摘要

The subject of invention is a method of encapsulating an active protein using electrodeposition techniques, characterised in that it comprises the following steps: (a) establishing a primary mesenchymal cell culture containing 2,000-5,000 source tissue cells and a serum-supplemented culture medium; (b) maintaining the cell culture established in step (a) for 280-340 hours until the culture surface is fully covered by the cultured cells; (c) obtaining a culture fluid from the above of the cultured cells; (d) purifying the culture fluid obtained in step (c) from cell debris and suspended cells by centrifuging said fluid with a force of 300 to 1200 x g; (e) transferring the upper liquid phase from above the sediment to a new vessel; (f) gently mixing the purified liquid phase obtained in step (e) with an aqueous solution of polyvinyl alcohol; (g) adding ethyl alcohol to the mixture obtained in step (f) while stirring continuously; (h) the material obtained in step (g) is deposited on the collector surface by means of electro spinning or electrospraying. Another subject of invention is an immunomodulating composition containing an active protein and a polymer, characterised in that it contains ethyl alcohol, wherein the active protein is a fibrous, fully water-soluble material containing proteins released by mesenchymal cells, including CCL2 at an amount from 0.56 to 5.62 ng/g of dry weight of the composition, and the polymer is an aqueous solution of polyvinyl alcohol. Another subject of invention is an use of the composition according to the invention for the preparation of a pharmaceutical composition for the treatment of atopic dermatitis in humans.