Method for multiplex detection of miRNA and miRNA detection kit using the same

摘要

The present invention relates to a method for simultaneous multiple detection of miRNA and a kit for detecting miRNA using the same, and more particularly, to a method for detecting miRNA using the same, comprising the steps of: synthesizing a plurality of cDNAs using a reverse transcription primer that hybridizes with a portion of the 3'' end of each of a plurality of target miRNAs; synthesizing a plurality of PCR templates by bidirectionally extending the plurality of reverse transcribed cDNAs with a plurality of extension primers that hybridize with a portion of the 3'' end of each; It relates to a simultaneous multiplex detection method of miRNA comprising: amplifying the plurality of PCR templates using a specific forward primer and a reverse primer, and detecting miRNA with a plurality of double-labeled probes. Since the miRNA simultaneous multiplex detection method of the present invention uses a shorter reverse transcription primer compared to a commercial miRNA detection kit, it is highly reactive in the reverse transcription step, thereby enabling accurate cDNA synthesis, and each reverse transcription primer for detecting a plurality of miRNAs; Since it is easy to design an extension primer, a primer set for PCR amplification, and a double-labeled probe for miRNA detection, a multiplex analysis capable of detecting a plurality of miRNAs at the same time is possible.