QUANTITATIVE DETECTION METHOD FOR SIX ANTIBIOTIC DRUG RESISTANT GENES IN AQUATIC PRODUCT

摘要

The present invention relates to the technical field of biology, and provides a quantitative detection method for six antibiotic drug resistant genes in an aquatic product. The method comprises: taking the total DNA of bacteria carried by an aquatic product to be tested as a template, respectively using a primer pair I to a primer pair VI to carry out fluorescent quantitative PCR amplification, and quantifying antibiotic drug resistant genes tetA, sul2, cmlA, qnrS, aac(6')-Ib, and blaPSE in said aquatic product according to the Ct value.