HIGH-COMPATIBILITY PCR-FREE LIBRARY BUILDING AND SEQUENCING METHOD

摘要

Disclosed is a PCR-free library building and sequencing method. The present invention provides a PCR-free high-throughput sequencing method, comprising the following steps: according to the size of a nucleic acid to be subjected to sequencing, performing or not performing a fragmentation treatment on the nucleic acid, so as to obtain a DNA fragment of a target size; preforming tail end repair and an A addition reaction; connecting to an adapter that includes a tag sequence; performing single-strand cyclization and rolling circle replication to form a DNA nanosphere; and performing sequencing on a machine.