Method for detecting phosphorylated alpha synuclein

摘要

Problem to be solved: to provide a method for detecting alpha synuclein using a capture antibody and reporter antibody.The capture antibody preferentially binds to a full length alpha synuclein (phosphatidylcholine) protein phosphorylated at residue 129 rather than non phosphorylated full-length alpha synuclein.11a5 antibody is an example of an appropriate capture antibody.Reporter antibodies bind to epitopes in residues 40-55 of alpha synuclein.23e8 antibody is an example of such an antibody.Since only one part of the alpha synuclein is phosphorylated, a high detection sensitivity is less than picomol.Diagram