Method for calibrating the observed volume of a nanoparticle tracking counting device based on light sheet

摘要

PROBLEM TO BE SOLVED: To properly calibrate a surveyed volume for particles of various types and sizes. A method of calibrating a darkfield microscope setting is disclosed. This method is to prepare a plurality of particle samples, each of which has a known concentration and particle size, wherein the plurality of particles have two or more particle sizes, and optionally two or more refractive indexes and two or more. Includes preparing, having a diluent for. For each of the plurality of samples, the sample is measured in the above settings, and the scattered light intensity and the number of particles are measured. From this data, the relationship between scattered light intensity, particle size, and calibrated / investigated volume can be determined. The calibrated and investigated volume is used to determine the proper particle size distribution in a given diluent. [Selection diagram] FIG. 11