RECOMBINANT STRAIN FOR PRODUCING L-AMINO ACID, CONSTRUCTION METHOD THEREFOR, AND APPLICATION THEREOF

摘要

Provided is a bacterium for producing L-amino acid. The bacterium has improved expression of a polynucleotide for encoding a protein represented by SEQ ID NO:3 and improved expression of a polynucleotide for encoding a protein represented by SEQ ID NO:31, and/or has mutations in bases at positions -45bp and -47bp of a promotor region represented by SEQ ID NO:57. Also provided are a polynucleotide, proteins encoded thereby, a recombinant carrier and a recombinant strain comprising a polynucleotide sequence, and a method for producing L-amino acid. The polynucleotide encodes a protein which is represented by SEQ ID NO:3 and has arginine at position 334 substituted by a terminator or encodes a protein which is represented by SEQ ID NO:31 and has tyrosine at position 592 substituted by phenylalanine, or is formed by mutations in bases at positions -45bp and -47bp of a promotor region represented by SEQ ID NO:57.