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HETEROZYGOUS CENH3 MONOCOTS AND METHODS OF USE THEREOF FOR HAPLOID INDUCTION AND SIMULTANEOUS GENOME EDITING
HETEROZYGOUS CENH3 MONOCOTS AND METHODS OF USE THEREOF FOR HAPLOID INDUCTION AND SIMULTANEOUS GENOME EDITING
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机译:杂合CENH3单像及其使用方法,用于单倍体诱导和同时基因组编辑
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摘要
Monocot plants heterozygous for centromeric histone 3 (CenH3) and optionally expressing gene editing constructs, for use in inducing haploids of a monocot target plant and optionally pass-through gene editing are provided. The monocot haploid inducer plants are typically composed of diploid plant cells having only one allele encoding a functional CENH3 protein. The diploid plant cells can also include, for example, one CenH3 allele encoding non-functional CENH3 protein. In some embodiments, the allele encoding non-functional CENH3 protein is a frameshift mutation, protein null allele, an RNA null allele, or a combination thereof. The monocot haploid inducer plant can also include gene editing machinery, such as a site-directed nuclease and optionally a guide RNA stably expressed by cells of the monocot plant. Methods of inducing formation of a target haploid monocot plant while optionally simultaneously modifying the target monocot plant' s genome are also provided.
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