Peptide-crosslinked Protein-imprinted Polymers And Preparation Method And Applications Thereof

摘要

A peptide-crosslinked protein-imprinted polymer, preparation method, and application thereof. One method comprises: 1) dissolving a main monomer, functional monomers, a peptide crosslinking agent, and a template protein in an aqueous solution to obtain a mixed solution; 2) adding an initiator or initiator system to the mixed solution to initiate the polymerization when the peptide crosslinking agent exists in a helix conformation to obtain a polymer; 3) eluting the template protein when the peptide chain exists in a coil conformation to obtain a peptide-crosslinked protein-imprinted polymer. The peptide crosslinking agent is a peptide with a polymerizable double bond at its both ends, and being capable of undergoing helix-coil transition. The polypeptide crosslinking agent is a polypeptide having an amino acid sequence which has a polymerizable double bond at its both ends, being capable of undergoing a helix-coil conformational transformation. The polypeptide cross-linked protein molecule-imprinted polymer disclosed in the invention not only can completely remove the template protein under mild conditions, but also can significantly improve the imprint effect of the protein molecule-imprinted polymer.