Scalable process for oncolytic rat parvovirus H-1 production and purification based on isoelectric point-based elimination of empty particles

摘要

The present invention provides a reproducible, effective and scalable process for the purification of (infectious) parvovirus H-1 particles. The purification process allows the separation of empty particles from particles containing a full genome and is compatible with large-scale H-1PV production for clinical applications.