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Prokaryote-based cell-free system for the synthesis of glycoproteins
Prokaryote-based cell-free system for the synthesis of glycoproteins
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机译:用于合成糖蛋白的基于原基科的细胞系统
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摘要
The present invention is directed to a cell-free system for producing a glycosylated protein. This system comprises an isolated oligosaccharyltransferase capable of transferring a glycan from a lipid carrier molecule to a glycoprotein target, one or more isolated glycans, where each glycan is linked to a lipid carrier molecule, and a glycoprotein target comprising one or more glycan acceptor amino acid residues or a nucleic acid molecule encoding said glycoprotein target. The present invention further relates to kits and methods for producing a glycosylated protein in this cell-free system.
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