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PROKARYOTE-BASED CELL-FREE SYSTEM FOR THE SYNTHESIS OF GLYCOPROTEINS
PROKARYOTE-BASED CELL-FREE SYSTEM FOR THE SYNTHESIS OF GLYCOPROTEINS
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机译:基于原核生物的无细胞糖蛋白合成系统
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摘要
The present invention is directed to a cell-free system for producing a glycosylated protein. This system comprises an isolated oligosaccharyltransferase capable of transferring a glycan from a lipid carrier molecule to a glycoprotein target, one or more isolated glycans, where each glycan is linked to a lipid carrier molecule, and a glycoprotein target comprising one or more glycan acceptor amino acid residues or a nucleic acid molecule encoding said glycoprotein target. The present invention further relates to kits and methods for producing a glycosylated protein in this cell-free system.
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