Disclosed is a rapid vitrification cryopreservation method of a strongylocentrotus intermedius embryo. Vitrification is realized by mixing dimethyl sulfoxide (DMSO), methanol (MeOH) and 1, 2-propylene glycol (1, 2-PG) with filtered and disinfected seawater, precooling for 15 minutes at the temperature of 2℃, and the volume concentrations of the dimethyl sulfoxide, the methanol and the propylene glycol are 1.0%-2.0%, 1.5%-2.0% and 1.5%-2.0% respectively; and the recovery method is realized by means of treatment such as 25℃ water bath thawing. The intact rate of the thawed strongylocentrotus intermedius embryo archenteron can reach 47.81%. The method has the advantages that the intact rate of the thawed embryo archenteron is high, operation is easy and the cost is low.
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