DEVICE FOR THE CRYOPRESERVATION OF A SAMPLE OF OVULES AND EMBRYOS BY VITRIFICATION

摘要

Device for the cryopreservation of a sample of ovules or embryos by vitrification comprising a support (1) having a single piece and comprising a slender fastening portion (2) configured to hold and label the sample and which joins with an intermediate portion (3) connecting with a tip (4), where said tip (4) has a distal end (5) of pointed shape and comprises a through hole (6) with an elliptical configuration comprising a maximum width of 0.6 mm intended to form a thin film of vitrification medium on which the ovules or embryos are deposited to vitrify by direct contact with liquid nitrogen. The device by its geometry, generates a tangential force taking advantage of the surface tension of the liquid to retain and envelop the cells, favoring even more to reach ultra-fast speeds of cooling and heating. (Machine-translation by Google Translate, not legally binding)