Recombinant expression vector for rapid screening of recombinant strains and its applications

摘要

The present invention provides a recombinant expression vector for rapidly screening high-expressing strains and a method for rapidly screening high-expressing strains. In the above method, an extrinsic red fluorescent protein (DsRed) and a signal peptide (AfMP1) that fixes the protein on the cell surface of Aspergillus fumigatus are fused and expressed, and this fusion gene (DsRed-AfMP1) is expressed by Trichoderma reesei. Integrate into the genome to construct a strain displaying the red fluorescent protein on the surface of Trichoderma reesei. By screening the Trichoderma reesei strain, which displays a red fluorescent protein on the surface, with a flow cytometer, it is possible to rapidly screen for genetic changes beneficial for improving cellulase activity. [Selection diagram] Fig. 1