Non-viral modification of mesenchymal stem cells

摘要

It is a non-viral for transfection of mesenchymal stem cells with a nucleic acid construct in which one or more functional genes are expressed, in certain embodiments high transfection efficiency, high copy number per cell, high cell viability, for an extended period of time. Described herein are methods that can provide for transient expression and/or a substantially unchanged pluripotent phenotype. In certain embodiments, these methods may be scalable and/or suitable for large-scale clinical production of modified mesenchymal stem cells. Also, the use of the transfected mesenchymal stem cells and populations of mesenchymal stem cells in methods for the treatment of diseases or disorders such as cancer using such transfected mesenchymal stem cells and kits associated therewith; Compositions are described in detail.