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CORONAVIRUS RAPID DETECTION KIT BASED ON S PROTEIN LIGAND AND ACE2 RECEPTOR COMPETITIVE CHROMATOGRAPHY
CORONAVIRUS RAPID DETECTION KIT BASED ON S PROTEIN LIGAND AND ACE2 RECEPTOR COMPETITIVE CHROMATOGRAPHY
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机译:基于S蛋白质配体和ACE2受体竞争色谱法的冠状病毒快速检测试剂盒
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摘要
A coronavirus rapid detection kit based on S protein ligand and ACE2 receptor competitive chromatography, comprising materials such as quantum dot labeled ACE2 protein, quantum dot labeled rabbit IgG, recombinant coronavirus spike protein S1, goat anti-rabbit IgG polyclonal antibody, an immunochromatographic test strip. The detection sensitivity is improved by means of quantum dot fluorescence labeling and multistage coupling amplification signals, the detection specificity is improved and the antibody research and development cycle is avoided by utilizing a ligand and receptor binding principle. Provided is the kit capable of rapidly detecting a coronavirus, and the biosafety in a detection process is guaranteed by establishing a virus inactivation system. The kit is suitable for detecting various biological samples and environmental samples such as oral mucosa liquid, respiratory tracts, whole blood, plasma, serum, and excrement, and can be applied to rapid detection of coronaviruses taking ACE2 as a receptor, such as SARS-CoV-2, SARS-CoV, and HCoV-NL63.
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