CONSTRUCTION METHOD FOR AND APPLICATION OF RECOMBINANT DRUG-RESISTANT BCG STRAIN

摘要

Provided is a construction method for a recombinant drug-resistant BCG strain, comprising: using M.bovis BCG as an original bacteria to screen a drug-resistant BCG strain having resistance to least one among streptomycin, levofloxacin, ethambutol, prothiaxamine, aminosalicylic acid and amikacin; and further inserting a sequence fragment that can express the related antigens Ag85b and Rv2628 that cause an immune response, so as to construct a recombinant drug-resistant BCG strain. The recombinant drug-resistant BCG strain can compete with Mtb for growth, thereby accelerating the death thereof, and in combination with a drug for treating tuberculosis, the therapeutic effect thereof for Mtb can be further strengthened.