NOVEL FLAVONE HYDROXYLASES, MICROORGANISM FOR SYNTHESIZING FLAVONE C-GLYCOSIDE COMPOUNDS, AND USE THEREOF

摘要

Provided are novel flavone hydroxylases, a microorganism for synthesizing flavone C-glycoside compounds, and use thereof. The present inventor obtains novel flavone hydroxylates PhF2H and PhF3'H by cloning, which belong to cytochrome P450 hydroxylates and have the function of hydroxylating specific positions of compounds. Further, the present inventor, by modifying the enzymes and combining a C-glycoside glycosyltransferase and assembly of a synthesis pathway of a flavone precursor, efficiently synthesizes flavone C-glycoside compounds such as orientin, isoorientin, vitexin and isovitexin, and related intermediates such as eriodictyol and 2-hydroxynaringenin in the synthesis pathway thereof in an artificial recombinant expression system.