RECOGNITION OF CELLULAR TARGET BINDING BY A BIOACTIVE AGENT USING INTRACELLULAR BIOLUMINESCENCE RESONANCE ENERGY TRANSFER

摘要

The present invention provides compositions and methods for the detection and analysis of intracellular binding of a cellular target to a bioactive agent. In particular, detecting and analyzing a bioactive agent tethered with a fluorophore, a cellular target fused with a bioluminescent reporter, or a portion, component, or subunit of a bioluminescent reporter, and the interaction of the cellular target with the bioactive agent therewith A method is provided herein.