LASER-INDUCED GRAPHENE ELECTROCHEMICAL IMMUNOSENSORS

摘要

Apparatus and methods of fabrication and use of a highly sensitive and label-free laser-induced graphene (LIG) electrode (10) functionalized with biorecognition agents (e.g. antibodies) (18) to electrochemically quantify a target species (e.g. foodborne pathogen Salmonella enterica serovar Typhimurium). In one example, the LIG electrodes are produced by laser induction on polyimide film (12) in ambient conditions, and circumvent need for high-temperature, vacuum environment, and metal seed catalysts commonly associated with graphene-based electrodes fabricated via chemical vapor deposition processes. After functionalization, the LIG biosensors detect a target species across a wide linear range with a low detection limit, and quick response time without need for sample pre-concentration or redox labeling. These LIG immunosensors displayed high selectivity by non-significant response to other bacteria strains, and demonstrate LIG-based electrodes can be used for electrochemical biosensing and immunosensing. One example for rapid, low-cost pathogen detection in food processing facilities before contaminated foods reach the consumer.