PLASMONIC CHIP, AND CANCER DISEASE DIAGNOSIS METHODS RESPECTIVELY EMPLOYING FLUORESCENT IMAGE AND RAMAN SPECTROSCOPY

摘要

[Problem] To provide: a plasmonic substrate; a method for observing fluorescent image of a candor related substance and a method for Raman spectroscopic analysis using the plasmonic substrate. [Solution] In the present invention, a cancer-related substance in blood or a biological sample can be detected in a selective manner, so it becomes possible to determine the occurrence of cancer by observation of fluorescent image of a crystal of the censor related substance or a coagulated state of the crystal on a plasmonic chip. In addition, the state of chemical modification of a histone tail can be determined by a Raman spectrum analysis, so that it becomes possible to detect occurrence of cancer at an earlier stage and to determine stages of progress of cancer. Furthermore, the location of the cancer-related substance aggregated on a substrate cannot be determined with naked eyes. Then, as a second aspect of the present invention, a method for diagnosing a cancer disease is provided, said method being characterized by firstly identifying the location of the region of a crystal by observing fluorescent image on a microscope, and then irradiating the crystal, with laser beam to analyze with respect to the chemical modification of a histone tail and a remodeling factor.