Method for producing synovial derived mesenchymal stem cell and manufacturing method of cell preparation for joint therapy

摘要

A method for producing synovial membrane mesenchymal stem cells capable of obtaining a sufficient amount of synovial membrane derived mesenchymal stem cells from synovial tissue, a method for producing a cell preparation for joint therapy using the above described method of production of synovial derived mesenchymal stem cells, synovial membrane mesenchymal stem cells Provide a cell preparation for joint therapy.A method for producing synovial derived mesenchymal stem cells from synovial tissue, treating the synovial tissue over an enzyme for more than two hours, and after mixing the enzyme A method for producing mesenchymal stem cells derived from the synovium, which can be obtained by washing the mesenchymal stem cells from the synovium by washing until the residual enzyme concentration in the supernatant reaches 0.5 ng / ml or less.No selection