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DETERMINATION AND QUANTIFICATION OF PROTEOSE PEPTONE CONTENT AND/OR BETA-CASEIN CONTENT AND NUTRITIONAL COMPOSITON WITH REDUCED BETA-CASEIN DERIVED PROTEOSE PEPTONE CONTENT
DETERMINATION AND QUANTIFICATION OF PROTEOSE PEPTONE CONTENT AND/OR BETA-CASEIN CONTENT AND NUTRITIONAL COMPOSITON WITH REDUCED BETA-CASEIN DERIVED PROTEOSE PEPTONE CONTENT
The present invention relates to a method for determining and quantifying β-casein derived proteose peptones and/or β -casein, said method comprising the steps of (i) providing a dairy-based product to be analysed; (ii) subjecting said product using liquid chromatography - mass spectrometry analysis; (iii) determining and/or quantifying said β -casein derived proteose peptones and/or β -casein in said product by detecting compounds of defined m/z values or deconvoluting the mass spectrometry spectra to calculate monoisotopic masses. The present invention also relates to nutritional compositions having a reduced content of β -casein derived proteose peptones and the uses hereof for e.g. treating, preventing and/or ameliorating abdominal pain in an infant.
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