APPARATUS AND METHOD FOR FAST VOLUMETRIC FLUORESCENCE MICROSCOPY USING TEMPORALLY MULTIPLEXED LIGHT SHEETS

摘要

A microscopy device comprises a continuous or pulsed wave laser light source; a pair of parallel mirrors configured to receive light from the light source and reflect an array of incoherent light sheets; a beam encoder (e.g., frequency modulation reticle, Hadamard basis, random modulation pattern) to segment the array of incoherent light sheets and encode each light sheet with a respective frequency in reciprocal space; a lens configured to direct the encoded light sheets towards a biological sample; and an image capturing device configured to receive a fluorescence signal from the biological sample.