Immunochromatographic test piece for extracting and measuring sugar chain antigen, which can control the development of a sample by impregnating a hydrophilic material with nitrite or a solid acid reagent

摘要

The present invention provides an immunochromatographic test piece for controlling the development of a sample on the immunochromatographic test piece, and an immunochromatographic test piece for appropriately controlling treatment with an acid reagent, nitrite and neutralizing reagent, and an immunochromatographic method using the test piece. intended to provide A sample pad to which a sample mixed with nitrite or acidic solution is added, a label region comprising a labeled antibody labeled with an antibody to the sugar chain antigen, and a detection region immobilized with the antibody to the sugar chain antigen; wherein the antibody-sugar chain antigen-labeled antibody complex is formed and the sugar chain antigen is measured, the immunochromatographic test piece having a region impregnated with a neutralizing reagent upstream of the label region, and the region impregnated with the neutralizing reagent Upstream, when using a sample mixed with nitrite, it has a region impregnated with a solid acid reagent, and when using a sample mixed with an acid solution, it has a region impregnated with nitrite. An immunochromatographic test piece, characterized in that a material having hydrophilicity is used in a region impregnated with nitrite or a solid acid reagent as a chromatography test piece.