If the spot positions placed on the board are misrecognized as positions of adjacent spots in a pattern, or if the board expands or deforms due to device drive, temperature control, etc., it becomes difficult to align the images. Become. Provided are a nucleic acid analysis substrate provided with a patterned spot portion forming a spot on which a biopolymer adheres and a random spot portion on the surface of the substrate, and an analysis method.
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